High-resolution characterization of cells and microorganisms based on the Coulter principle

How to measure the volume and size of cells, as well as the numeric, surface and volume distribution of all conceivable cell types

Experience state-of-the-art cell characterization: the patented digital pulse processing (DPP) technology that the Multisizer 4e uses has implemented the classic impedance measurement method, also called “Coulter Principle” after its inventor, fully digitally.

The Multisizer 4e is one of the most accurate and flexible cell and particle characterization instruments on the market, boasting an unparalleled sizing range of 0.2 to 1600 μm. The new 10 μm aperture allows users to study sub-cellular and micro-particles as small as 200 nm, while the advanced noise reduction system for small apertures improves measurement accuracy. But also large objects or particles sized well over 100 µm can be volumetrically detected and accurately counted.

The generated particle data are all processed by the DPP technology and can be stored and analyzed once more at a later time. This technology provides ultra high resolution and accuracy unattainable through any other technologies, being able to detect a single particle in a 1 ml sample with the optimal instrument configuration. Analysis results do not depend on particle shape, structural, or optical properties. Carry on reading to find out more, or contact us if you have any questions!

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Advantages of characterizing cells and microorganisms with the Multisizer 4e:

Maximum flexibility in measuring a wide range of cell types thanks to a sizing range of 0.2 – 1600 µm
Reliable determination of the cells’ optimal harvesting time
Early detection of differences in the mean cell volume (MCV) during experiments
Later recalculation of particle distribution & observation of each individual particle possible
Measurement of raw and modified polymeric particles

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Intelligent features that simplify the application

The extended measuring range of 2 to 80% of the aperture’s diameter means the aperture doesn’t need to be changed as often, giving more options for analysis. Any capillary blockages are detected electronically and removed using a patented technology. A barcode scanner quickly enters information on samples, electrolytes, aperture size, and sample beaker digitally. Liquid levels in the vessels are automatically monitored and warnings displayed when electrolytes need to be replenished or waste containers emptied. Stirring speed and direction can be selected to ensure an even particle distribution in a large-volume suspension. Thanks to the instrument’s new design, your sample is protected from dust and dirt at all times. In addition, its resistance to solvents makes the Multisizer 4e easy to disinfect.

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Cell characterization based on the Coulter principle and DPP is suitable for:

Bioprocess engineering, life sciences, academic & biopharmaceutical research
Any cell type: bacteria, yeasts, fungi, plant and mammalian cells, including animal sperm, animal blood etc.
Characterization of sub-cellular and micro-particles
Cell size determination, cell counting and particle distribution
Determination of the mean corpuscular volume (MCV)

This is only a selection of the possible applications. You’re interested in a special application area where the impedance measurement method of the Multisizer 4e Coulter counter can help you? Or where you are currently already facing a challenge? Then simply request further information and let us know your specific application in the comments field.

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Accelerate bioprocess development with microbioreactors for high-throughput fermentation

A conventional scale-up process from early screening experiments to production scale can take days or even weeks. Imagine how time-consuming it would be to run 48 parallel fermentations in larger fermenters …. the BioLector microbioreactors let you achieve this throughput with only two hours of work.

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